combination antioxidant effect of erythropoietin and melatonin on renal ischemia-reperfusion injury in rats

Authors

nasser ahmadiasl drug applied research center, tabriz university of medical sciences, medical faculty, tabriz, iran

shokofeh banaei department of physiology, tabriz university of medical sciences, tabriz, iran

alireza alihemmati department of histology & embryology, tabriz university of medical sciences, medical faculty, tabriz, iran

abstract

objective(s): renal ischemia reperfusion (ir) contributes to the development of acute renal failure (arf). oxygen free radicals are considered to be principal components involved in the pathophysiological tissue alterations observed during renal ir. the purpose of this study was to investigate the effect of co-administration of melatonin (mel) and erythropoietin (epo), potent antioxidant and anti-inflammatory agents, on ir-induced renal injury in rats.   materials and methods: wistar albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 hr reperfusion. mel (10 mg/kg, ip) and epo (5000 u/kg, ip) were administered prior to ischemia. after 24 hr reperfusion, following decapitation, renal samples were taken for the determination of malondialdehyde (mda), superoxide dismutase (sod), catalase (cat), glutathione peroxidase (gpx) levels and histological evaluation. the level of urea was measured in serum samples. results: ischemia reperfusion significantly increased urea, and mda levels, and decreased cat and sod activities. histopathological findings of the ir group confirmed that there was renal impairment in the tubular epithelium. treatment with epo and mel markedly decreased urea level and increased sod and gpx activities. conclusion: treatment with epo and mel had a beneficial effect on renal ir injury. these results may show that the co-administration of mel and epo cannot exert more beneficial effects than either agent alone.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Combination Antioxidant Effect of Erythropoietin and Melatonin on Renal Ischemia-Reperfusion Injury in Rats

  Objective(s): Renal ischemia reperfusion (IR) contributes to the development of acute renal failure (ARF). Oxygen free radicals are considered to be principal components involved in the pathophysiological tissue alterations observed during renal IR. The purpose of this study was to investigate the effect of co-administration of melatonin (MEL) and erythropoietin (EPO), potent antioxidant and ...

full text

Combination Antioxidant Effect of Erythropoietin and Melatonin on Renal Ischemia-Reperfusion Injury in Rats

OBJECTIVE(S) Renal ischemia reperfusion (IR) contributes to the development of acute renal failure (ARF). Oxygen free radicals are considered to be principal components involved in the pathophysiological tissue alterations observed during renal IR. The purpose of this study was to investigate the effect of co-administration of melatonin (MEL) and erythropoietin (EPO), potent antioxidant and ant...

full text

combination anti-apoptotic effect of erythropoietin and melatonin on ischemia reperfusion-induced renal injury in rats

renal ischemia-reperfusion (ir) contributes to the development of acute renal failure (arf). oxygen free radicals are considered to be principal components involved in the pathophysiological tissue alterations observed during renal ir. the purpose of this study was to investigate the combination effect of melatonin (mel) and erythropoietin (epo), which are a potent antioxidant and anti-apoptoti...

full text

The anti-inflammatory effect of erythropoietin and melatonin on renal ischemia reperfusion injury in male rats.

PURPOSE Renal ischemia reperfusion (IR) is an important cause of renal dysfunction. It contributes to the development of acute renal failure (ARF). The purpose of this study was to investigate the anti-inflammatory effect of erythropoietin (EPO) and melatonin (MEL), which are known anti-inflammatory and antioxidant agents, in IR-induced renal injury in rats. METHODS Male Wistar Albino rats we...

full text

ameliorative effect of recombinant human erythropoietin and ischemic preconditioning on renal ischemia reperfusion injury in rats

conclusions pretreatment with epo and application of ipc significantly ameliorated the renal injury induced by bilateral renal ir. however, both treatments attenuated renal dysfunction and oxidative stress in kidney tissues. there were no significant differences between pretreatment with epo or application of ipc. materials and methods twenty four male wistar rats were allocated into four exper...

full text

comparison of the protective effects of erythropoietin and melatonin on renal ischemia-reperfusion injury

conclusions treatment with epo and mel had a beneficial effect on renal ir injury. the results may also indicate that mel protects against morphological damage better than epo in renal ir injury. results ischemia-reperfusion significantly decreased the observed hb and hct values. the histopathological findings in the ir group confirmed that there was an increase in the hyaline cast and thickeni...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۶، شماره ۱۲، صفحات ۱۲۰۹-۱۲۱۶

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023